Abstract
The current study aimed to adopt a method for inducing callus cells and regenerating the important common red bean using different types of growth regulators such as N6-benzylaminopurine (BAP), Naphthalene acetic acid (NAA), and Thidiazuron (TDZ). Different types of common bean pinto cultivar explants, such as internodes, cotyledons and roots, were inoculated on Murashige and Skoog medium (MS) provided with different combinations of plant growth regulators, including 1- BAP (5 mg/l) 2-BAP (4.5 mg/l) NAA (0.5 mg/l), 3- BAP (4.5 mg/l), and TDZ (0.1mg/l). Callus was initiated on MS culture medium supplied with 5 mg/l BAP for all explants (internodes, cotyledons, and roots) at 50, 20, and 10% respectively, while adding NAA with 0.5mg/l showed a low percentage of callus (30%) only in the internode explants. Optimum results were obtained by growing the internodes on MS medium with 4.5 mg/l BA and either 0.5 mg/l NAA or 0.1 mg/l TDZ, transplanting the derived shoots into internodes and cotyledons with 70 and 10% respectively. This study concludes that the internodes as explants have the best growth results.
Highlights
Mexico bean (Phaseolus vulgaris L.) Pinto cultivar is an economically important crop that is considered as one of the main cultivated legumes for human consumption in Latin America, Africa, and Asia
In vitro indirect regeneration has been achieved, few studies reported the indirect regeneration of P. vulgaris and the regeneration of the shoot from callus is quite little, which highly depends on cultivars [9, 10]
An efficient and reproducible protocol of the regeneration was achieved through organogenesis from cotyledonary nodes that were arisen from seedlings
Summary
Mexico bean (Phaseolus vulgaris L.) Pinto cultivar is an economically important crop that is considered as one of the main cultivated legumes for human consumption in Latin America, Africa, and Asia. The majority of protocols of common bean regeneration are relied on either the direct regeneration or shoot initiation from different explants, such as apical shoots and roots, cotyledonary nodes, internodes, petioles, etc [7, 8]. Explants of red pinto bean (internodes, cotyledon, and roots) were cultured on MS medium provided with different combinations of plant growth regulators, 1- BA (5 mg/l) 2- BA 4.5mg/l and 0.5 mg/l NAA 3- BA 4.5mg/l,0.5mg/l NAA and 0.1mg/l TDZ.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
