Abstract
Various combinations of exogenously applied growth regulators supported callus growth to different extents in cultures of leaf and tuber explants of the tetraploid potato cv. Bintje ( Solanum tuberosum L.). Various strains of Agrobacterium tumefaciens differing in induction of endogenous hormonal activities, induced tumours of varying habitus and frequency on tuber dics. The ploidy levels of cells from tissues were determined by means of flow cytometry. In the original explants, varying degrees of polyploidization were observed. Moreover, upon ageing of explanted leaf tissues, rapid endopolyploidization occurred. The DNA histograms of cell populations of calli or tumours were similar, irrespective of hormonal regime and explant source. Within 1 month of growth, a stable pattern of polyploidization was reached, the DNA histograms resembling the patterns found in tuber tissues and in aged leaf explants, cultured in the absence of plant growth regulatory substances. The factors influencing genetic stability, are attributed to predominant genotype effects.
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