Abstract
Callus formation and somatic embryogenesis in sugarcane (Saccharum spp L.) using various concentrations of 2, 4-D and RAPD analysis of regenerated plants
Highlights
Introduction profitable crop cultivated in74 countriesSugarcane (Saccharum spp L.) hybrid is a around the globe [1]
Buffer solution genomic Deoxyribonucleic acid (DNA) extraction Total genomic DNA was separated from samples of young leaf tissue of all the three varieties Bl4, NIA-2010, NIA-2011; three specimens of that recovered from somatic embryo callus were taken from field plantlets' and determined through nodal shoot multiplication and somatic embryogenesis from every accession
[19, 28] reported that some random amplified polymorphic DNA (RAPD) bands were acquired in sugarcane families, showing that RAPD bands could effectively be utilized as genetic markers
Summary
Callus formation and somatic embryogenesis in sugarcane (Saccharum spp L.) using various concentrations of 2, 4-D and RAPD analysis of regenerated plants. College of Resources and Environmental Science, State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agriculture University 210095, P.R , Nanjing-China
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