Abstract

Changes taking place at the base of stem cuttings of Douglas-fir during callus formation and root initiation are described. Material was taken from nine sources, including trees of different ages and with different histories of rooting response. Gross morphology and anatomy of the cutting bases are described using serial longitudinal sections of samples taken on a weekly basis over a 4-month period. The basal callus proliferated from the lowest cells of the vascular cambium and a complex pattern of differentiating vascular tissues arose within the callus mass. The root primordia arose in this callus in association with the differentiating phloem and wound cambium. Variation in callus formation and root initiation is described both within and between clones.

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