Abstract
Protoplasts were isolated from cotyledons of melon (Cucumis melo L.) seedlings grown in vitro. When young unexpanded cotyledons were a source material, 60% of protoplasts were viable and 40% of them had divided after 7 days of culture. Some of the protoplasts continued to divide to form callus colonies, and the differentiation of roots, but not of shoots, was induced in the protoplasts-derived calli.
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