Calling amplitude flexibility and acoustic spacing in the territorial frog Allobates femoralis

Abstract

Most male frogs produce calls to attract females and repel rivals. The transmission of these calls can be affected by many acoustic and environmental characteristics, which can influence the detection and decoding of the signal by the receiver. Calling-perch height has a strong influence on sound propagation and acoustic spacing with neighboring males, but how frogs optimize their calling behavior in this context is poorly understood. In this study, we investigated if and how frogs can adjust the calling energy in the context of acoustic spacing. Our aim was to evaluate the relationship between calling-perch height, nearest-neighbor distance, and sound-pressure level in the brilliant-thighed poison frog Allobates femoralis. We found that frogs flexibly adjust the calling amplitude according to the calling-perch height without affecting the effectiveness signal propagation. Accompanying signal propagation experiments demonstrated that calls produced with lower amplitude from higher perches propagate similar to louder calls from the ground. Our results suggest an adjustment to the hypothesis of a general positive effect of calling-perch height on signal effectiveness, where highly territorial frogs adjust their calling behavior to reduce energy expenditure and optimize acoustic communication with conspecifics.Significance statementIn acoustically communicating species, sound propagates better when broadcasted from elevated positions. However, callers may adjust their calling behavior to optimize the sound transmission under ecological constraints. By using a correlative and manipulative approach, we show in a poison frog model that males can adjust their calling amplitude according to the calling-perch height. We then discuss that this calling adjustment optimizes the acoustic spacing between conspecific males and reduces energy consumption.