Abstract
The product H (70-80U./mg.), obtained from hog pancreas by the method using Acrinol (2-ethoxy-6, 9-diaminoacridinium lactate), was purified further by zone electrophoresis using starch as a carrier (Procedure VIII). In an electrophoresis box of 40×10×1.5cm., using veronal buffer, a constant current of 2mA/cm2 was passed for 15-22 hours. Separated components were cut out with starch, extracted with distilled water, and the solution was lyophilized. The effective portion was purified to an extreme purity of 570U./mg. and this product was found to be a homogeneous unity from paper electrophoretic and ultra centrifugal analyses. The purity of each zone obtained by this method agreed with the values estimated from paper electrophoretic analysis. This method showed good reproducibility in repeated experiments and recovery of potency was comparatively good.
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