Abstract
The presentation deals with the calibration for a biological acoustic impedance microscope. A block of tissue was placed on a plastic substrate, and an acoustic pulse focused on the interface was transmitted from the rear side of the substrate. The reflection was received by the same transducer and interpreted into local acoustic impedance. An acoustic impedance image was obtained by mechanically scanning the transducer. A reference material was simultaneously observed in order to perform calibration. A water droplet was employed as a reference material, however, it often entered into the interface between the substrate and tissue, leading to the reduction of the image quality. Therefore, several kinds of compounds were investigated. Silicone rubber was relatively homogeneous and stable in acoustic impedance, after a certain period of curing time. However, its dispersion with the scale of focal spot size was as large as 0.02 Ns/cm3, suggesting that a sufficient number of points should be measured and aver...
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