Abstract
A calibration procedure for a Clark-type oxygen electrode over a wide range of temperatures is described. The autoxidation of duroquinol (2,3,5,6-tetramethyl-1,4-benzenediol) was used to verify the electrode's ability to accurately sense the total amount of dissolved O 2 in an aqueous buffer. Electrode response time was measured by using oxygenated ethanol to deliver a rapid increase in O 2 concentration to the reaction medium. An oxygen-producing system (spinach thylakoids) was utilized to test the range of O 2-evolution rates able to be sensed. It was concluded that a Clark-type oxygen electrode has the absolute sensitivity, rapidity, and range necessary to accurately track rates of O 2 production or consumption from 5 to 45°C.
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