Abstract
A calculator program and instructions are presented for facilitating the preparation of single cell suspensions to be used in cell culture systems. The program has been written for the Texas Instruments Inc. TI-59 programmable calculator with printer. The instructions provided are intended for the novice with no previous programming experience and only a basic understanding of the calculator operation. The program provides the appropriate volume and cell concentration of the inoculum cell suspension for a given set of culture parameters. It also provides options for culture initiation when the cell yield in the concentrated suspension is insufficient to satisfy the parameters selected.
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