Abstract
Plant growth and productivity is negatively affected by different stresses. Most stresses trigger calcium signals that initiate acclimation responses in plants. The multigene family of plant calcium-dependent protein kinases (CPKs) functions in multiple stress responses by transducing calcium signals into phosphorylation events. This work reports that the OsCPK10 isoform positively mediates tolerance to different stresses in rice plants by enhancing their antioxidant capacity and protecting them from reactive oxygen species (ROS) damage, with the uncontrolled generation of ROS being a common feature of these stresses. Here, we show that the constitutive accumulation of an HA-tagged OsCPK10 full-length protein enhances the hydrogen peroxide detoxifying capacity of rice plants during desiccation. This is achived by modulating the accumulation of catalase proteins, which reduces the extent of lipid peroxidation and protects the integrity of cell membranes, resulting in drought tolerance. OsCPK10HA accumulation also confers blast disease resistance by interfering with fungal necrotrophic growth via a reduction in the accumulation of hydrogen peroxide. Furthermore, we show by bimolecular complementation assays that OsCPK10 is a plasma membrane protein that physically interacts in vivo with catalase A. OsCPK10 therefore appears to be a good molecular target to improve tolerance to abiotic stresses as well as to blast disease, which limit rice crop productivity.
Highlights
Rice is the staple food for half of the world population
Immunodetection using anti-catalase antibodies showed an immunoreactive polypeptide around 55 kDa, with a higher intensity in the air-dried OsCPK10HA leaves than in wild-type or empty vector leaves (Fig. 3F). These results show that the accumulation of OsCPK10HA in rice plants leads to increased catalase accumulation in response to drought stress
This study reports that the OsCPK10 isoform of the calcium-dependent protein kinases (CPKs) family mediates drought tolerance, as well as blast disease resistance, in rice plants
Summary
Rice is the staple food for half of the world population. rice, as with other crops, is exposed to numerous environmental stresses that constrain its growth and development, culminating in harvest losses. Blast disease, caused by the ascomycete fungus Magnaporthe oryzae, is one of the most devastating diseases affecting rice crop worldwide (Dean et al, 2012). This fungus can infect all plant parts provoking its death in juvenile stages or causing total grain loss in panicle infections. In the context of global climate change, these negative environmental factors may be more pronounced and damaging For this reason, Abbreviations: ABA, abscisic acid; CPKs, calcium-dependent protein kinases; dpi, days post-infection; EV, empty vector; H2O2, hydrogen peroxide; hpi, hours post infection; MDA, malondialdehyde; MV, methylviologen; ROS, reactive oxygen species
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