Calcium uptake by cultured bone cells: The role of phosphate, calcitonin and 1,25-(OH)2 D3

Abstract

Our data present evidence that in cultured bone cells 1) A rise of phosphate concentration in the medium and the subsequent rise of theintra cellular phosphate content stimulate calcium and phosphate uptake by bone cells and enhance the calcitonin effect, which seems to be dependent on the intracellular concentration of phosphate. 2) Calcitonin predominantly influences the intracellular shift of calcium. 3) 1,25-(OH)2 D3 has a direct effect on cultured bone cells: it increases the calcium and phosphate turnover and, therefore, stimulates the calcitonin action.