Calcium transients evoked by ATP in the cells of the mouse organ of Corti

Abstract

Event Abstract Back to Event Calcium transients evoked by ATP in the cells of the mouse organ of Corti Máté Aller1*, Tamás Horváth1, Gábor Polonyi2, Ádám Fekete1, György Halmos3, Attila Heinrich1, Beáta Sperlágh1, Balázs Lendvai4, E. S. Vizi1 and Tibor Zelles1 1 HAS, Institute of Experimental Medicine, Hungary 2 Semmelweis University, Department of Oto-rhino-laryngology, Hungary 3 University Medical Center , Department of Otolaryngology, Netherlands 4 Gedeon Richter Ltd., Hungary The process of converting the mechanical sound waves into electrical signals is developed by the co-work of functionally different subunits of the inner ear. The organ of Corti is the effective transducer, where the functions of the different cell types are modulated by ATP, which generates [Ca2+]i changes in these cells. We have developed a method of fluorescence calcium imaging in acute hemicochlea preparation, using dissected mature mouse cochlea that keeps the original tissue organization. After bulk loading the preparation with the calcium dye Fura-2/AM, we could evoke repeatable calcium transients in the inner hair cells, pillar cells, Deiters cells, and Hensen cells by perfusion of ATP. Here, we show that simultaneously measured basal [Ca2+]i differs in the specialized cells of the organ of Corti of 15-21 days old mice. We observed the lowest basal [Ca2+]i in Deiters cells (47 nM ± 3 nM, n=87), and the highest in Hensen cells (87 nM ± 5 nM, n=71). Application of 50M ATP caused calcium transients with the lowest peak in inner hair cells (103 nM ± 9 nM, n=35), and the highest in Hensen cells (245 nM ± 13 nM, n=71). The response was dose-dependent in the applied 1M to 100M ATP range. In order to get more information about the purinergic receptors involved, we also applied ATP during a perfusion of PPADS, and performed RT-PCR analysis of P2X and P2Y receptors in the organ of Corti. To specify whether these calcium transients are connected more to internal stores or they are depending on the external source, we applied CPA, the specific inhibitor of Ca2+-ATPase of the internal stores, and calcium free buffer together with the ATP. Remarkable differences could be observed between the different cell types. By using nifedipine, we also found that L-type calcium channels possibly plays a role in the ATP evoked calcium transients in the Hensen cells. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Sensory and motor systems Citation: Aller M, Horváth T, Polonyi G, Fekete Á, Halmos G, Heinrich A, Sperlágh B, Lendvai B, Vizi ES and Zelles T (2010). Calcium transients evoked by ATP in the cells of the mouse organ of Corti. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00060 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Apr 2010; Published Online: 21 Apr 2010. * Correspondence: Máté Aller, HAS, Institute of Experimental Medicine, Budapest, Hungary, allerm@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Máté Aller Tamás Horváth Gábor Polonyi Ádám Fekete György Halmos Attila Heinrich Beáta Sperlágh Balázs Lendvai E. S Vizi Tibor Zelles Google Máté Aller Tamás Horváth Gábor Polonyi Ádám Fekete György Halmos Attila Heinrich Beáta Sperlágh Balázs Lendvai E. S Vizi Tibor Zelles Google Scholar Máté Aller Tamás Horváth Gábor Polonyi Ádám Fekete György Halmos Attila Heinrich Beáta Sperlágh Balázs Lendvai E. S Vizi Tibor Zelles PubMed Máté Aller Tamás Horváth Gábor Polonyi Ádám Fekete György Halmos Attila Heinrich Beáta Sperlágh Balázs Lendvai E. S Vizi Tibor Zelles Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.