Abstract
Calcium-sensing receptors (CASR) are a dimeric family of C-class G-protein-coupled receptors that play a crucial role in bone and mineral metabolism by regulating parathyroid hormone (PTH) secretion, skeletal development, and urinary Ca2+ excretion. Genetic factors mainly impact bone mineral density (BMD). However, many variable factors may affect bone health, including physical activity. The aim of our study was to investigate the potential associations between calcium-sensing receptor gene polymorphisms (CASRV1 and CASRV2) and the level of physical activity in adult males from Lower Silesia, a region in the south of Poland. A total of 428 adult male inhabitants of Lower Silesia were included in the study. Their physical activity was evaluated using the International Physical Activity Questionnaire. The CASRV1 (rs 1801725, G>T, A986S) and CASRV2 (rs 761486, T>G, non-coding region) polymorphisms were determined using polymerase chain reaction (PCR) and mini-sequencing. The polymorphisms were identified with GeneScan software ver. 3.1.2. We did not observe any statistically significant differences between the total energy expenditure (total MET) and the CASRV1 and CASRV2 polymorphisms. We did not find any association between the level of physical activity and the frequency of genotypes at the polymorphic locus of the calcium-sensing receptor genes CASRV1 and CASRV2. We found that the number of hours the subjects spent in a sitting position was unrelated to the genotypes at the polymorphic locus of the calcium-sensing receptor gene CASRV1. Based on our studies, we concluded that there were no associations between CASR and physical activity in the men inhabiting Lower Silesia in Poland. Our results do not suggest any influence of the assessed genetic factors in the population variability of the level of physical activity of adults.
Highlights
Regular physical activity is one of the most effective means of maintaining good physical and mental health in humans
We evaluated two polymorphisms of the Calcium-sensing receptors (CASR): CASRV1 and CASRV2
We investigated the calcium-sensing receptor gene polymorphism CASRV1; the frequency of the G allele was 80.5%, while that of the T allele was 19.5%
Summary
Regular physical activity is one of the most effective means of maintaining good physical and mental health in humans. Multiple studies investigating the impact of physical activity on health have been funded by organizations such as the European Union, the World Health Organization, and the International Olympic Committee. Researchers have been investigating the molecular determinants of spontaneous locomotor activity in animals (Lerman et al, 2002; Tou and Wade, 2002; Turner et al, 2005; Lightfoot et al, 2008). A number of publications has recently suggested that the level of physical activity in humans is significantly affected by genetic factors (Joosen et al, 2005; Stubbe et al, 2005, 2006; Lightfoot, 2011; Mitchell et al, 2016; Herbert et al, 2018). Studies on the human model conducted so far have revealed associations between the level of physical activity and the DNA sequence variability of the BDNF (brain-derived neurotrophic factor) gene (Bryan et al, 2007), the DRD2 (dopamine receptor D2) gene (Simonen et al, 2003), the LEPR (leptin receptor) gene (Stefan et al, 2002), the ACE (angiotensin-converting enzyme) gene (Winnicki et al, 2004), the MC4R (melanocortin-4 receptor) gene (Loos et al, 2005), and the CASR (calcium-sensing receptor) gene (Lorentzon et al, 2001)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
