Abstract
The properties of calcium transport in microsomes and the effect of inositol 1,4,5-trisphosphate (IP 3) on accumulated calcium were studied in rat thymocytes. Active calcium transport shows an apparent affinity constant for calcium of 0.2 ± 0.01 μM and a maximal velocity of 2.3 ± 0.6 nmol/mg/30 min (mean ± SD). IP 3 was able to induce release of calcium only in the absence of oxalate. At 6 μM ambient free calcium, half-maximal effect of IP 3 was attained at 2 μM and maximal calcium release was produced by IP 3 concentrations over 5 μM. Barium and strontium did not modify calcium uptake by microsomes but markedly inhibited the action of IP 3.
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