Calcium Preconditioning in Human Myocardium

Abstract

Background. Ischemic stress and other protein kinase C (PKC)–linked receptor stimuli can induce rapid cardiac protection against ischemia-reperfusion injury. We and others have demonstrated that exogenous calcium (Ca 2+) pretreatment confers PKC-mediated cardiac functional and infarct protection in animal models, but it remains unknown whether Ca 2+ preconditioning confers similar postischemic functional protection in human myocardium, and, if so, whether the mechanism is mediated by PKC. We postulated that Ca 2+ preconditioning confers ischemic tolerance to human myocardium by a PKC-dependent mechanism. Methods. Human atrial trabeculae were suspended in organ baths and paced at 1 Hz, and force development was recorded. After 90 minutes of equilibration, all trabeculae were subjected to ischemia (45 minutes) and reperfusion (120 minutes). Exogenous CaCl 2 (3.0 mmol/L for 5 minutes) or vehicle (saline solution) was administered before simulated ischemia, with or without concurrent PKC inhibition (bisindolylmaleimide I, 150 nmol/L). Results. Ischemia-reperfusion resulted in decreased postischemic developed force, Ca 2+ preconditioning protected human myocardium against ischemia-reperfusion injury ( p < 0.05 versus control ischemia-reperfusion), and concurrent PKC inhibition abolished the salutary effect of Ca 2+ preconditioning in human myocardium ( p < 0.05 versus Ca 2+ preconditioning). Conclusions. Preconditioning with Ca 2+ represents a potent means of accessing PKC-mediated protection of the human myocardium against ischemia-reperfusion injury.