Abstract
BackgroundEstradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore (CI) A23187 to synthesize PGF2α.ResultsTreatment with E2in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF2α was better stimulated with A23187 at concentrations of 10-6 and 10-5 mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/mL, while for cultures treated with E2, A23187, or a combination of E2 and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/mL, respectively.ConclusionsTreatment with A23187 tended to stimulate PGF2α production. In the presence of E2, A23187 significantly stimulated PGF2α synthesis. It appears that A23187 potentiates the effects of E2 with respect to synthesis of endometrial PGF2α in cattle.
Highlights
Estradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release
We sought to evaluate the capacity of E2, when administered in vivo, to stimulate PGF2α synthesis in endometrial explants incubated with a calcium ionophore (CI), melittin or oxytocin
PGF2α synthesis was similar among untreated explants (24.7 ± 4.6 pg/mL/mg of tissue) and explants treated with OT (28.2 ± 4.6 pg/mL/mg of tissue), melittin (26.9 ± 5.0 pg/mL/mg of tissue) or A23187
Summary
Estradiol (E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α (PGF2α) release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore (CI) A23187 to synthesize PGF2α. IP3 binds to receptors on the endoplasmic reticulum, The main goal of our study was to determine the ability of endometrial explants and cells to synthesize PGF2α. We sought to evaluate the capacity of E2, when administered in vivo, to stimulate PGF2α synthesis in endometrial explants incubated with a calcium ionophore (CI), melittin or oxytocin. We sought to determine the CI dose that was capable of stimulating the synthesis of PGF2α in cultured endometrial cells, and to evaluate the capacity of bovine endometrial (BEND) cells to synthesize PGF2α following treatment with E2 and/or a CI
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