Abstract
The periodontal pathogen Porphyromonas gingivalis modulates epithelial cell signal transduction pathways including Ca 2+ signaling, and internalizes within the host cell cytoplasm. Since nuclear and cytoplasmic [Ca 2+] increases can induce different host cell responses, P. gingivalis-related [Ca 2+] changes in these compartments were measured by digital fluorescent imaging microscopy. Non-deconvolved and deconvolved fura-2 images showed that P. gingivalis exposure caused human gingival epithelial cells cultured in physiologic [Ca 2+] levels to undergo sustained oscillations of [Ca 2+] in nuclear and cytoplasmic spaces. However, P. gingivalis invasion was not tightly correlated with intracellular [Ca 2+] oscillations, since invasion could significantly precede, or even occur in the absence of, oscillations. [Ca 2+] oscillations required a Ca 2+ influx, which was completely inhibited by La 3+ or 2-APB (2-aminoethoxydiphenyl borate), indicating Ca 2+ entry was via a Ca 2+-permeable channel. Ca 2+ entry was likely not via a store-operated channel, since Ca 2+ release from intracellular stores was not observed during cellular uptake of P. gingivalis. Hence, uptake of P. gingivalis in gingival epithelial cells induces oscillations in nuclear and cytoplasmic spaces by activating a Ca 2+ influx through Ca 2+ channels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
