Abstract
Protocols were defined that enable patch-clamp studies of the ∼107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10 −7 M or lower during mitochondrial isolation, no activity was observed at low voltage (±60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of ∼ 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10 −9 to 10 −5 M decreased the probability of opening for the MCC and ∼107 pS activities.
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