Abstract
Monolayer cultures of mouse adrenal cortex tumors have been used to investigate the effects of Ca2+ on the binding of 125I-labelled s1-24-ACTH peptide to the cell, ACTH-stimulated cyclic-AMP and steroid biosynthesis. Stimulation with ACTH results in increased intracellular cyclic-AMP levels and release of cyclic-AMP into the medium. The specific activities of intracellular and extracellular cyclic-AMP produced by cells incubated with 3H-adenine are the same, suggesting that they are products of a common pool of ATP. ACTH-stimulated cyclic-AMP release continues after intracellular cyclic-AMP levels have decreased and reflects continuing cyclic-AMP biosynthesis. In incubations of two hours or less with 5.0 mM Ca2+, maximum steroidogenesis occurs with 0.1 milliunit/ml ACTH. Further increases in cyclic-AMP production are elicited with higher concentrations of ACTH. In the absence of Ca2+, both cyclic-AMP and steroid syntheses are attenuated, maximum steroidogenesis requiring > 10 milliunits/ml ACTH.The propor...
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