Calcium Ionophore Supplementation in In vitro Fertilization Medium towards Fertilization Rates of Kacang Goats (Capra hircus)

Abstract

In vitro fertilization is assisted reproduction technology that can overcome infertility problems. This method can produce embryos with high quality in large quantities. In its application, the success rate of IVF is still relatively low. This is due to the poor quality of oocytes and spermatozoa, which can cause IVF failure. The absence of oocyte activation during fertilization also results in failure of fertilization. Calcium ionophore is known to be able to increase the oocyte activation process. This study aims to prove that Calcium Ionophore can increase the rate of fertilization in goat oocytes in vitro. This study was divided into two groups: the control group was fertilized oocytes using a medium without Calcium ionophore supplementation, the treatment group was fertilized oocytes using a medium supplemented with Calcium ionophore at a dose of 5.2 µl/ml. Fertilization rates are observed based on the formation of zygote or two pronuclear (2PN) in the cytoplasm of oocytes and observed under an inverted microscope. The data obtained were analyzed using the T test. The results of the study after being analyzed statistically showed that there were significant differences between the control group and the treatment group (P<0.05). Fertilization rates in the treatment group (93.12±13.7) showed higher results than the control group (65.96±28.36). Based on these results it can be concluded that calcium ionophore supplementation at a dose of 5.2 µl/ml in the fertilization medium can increase oocyte activation thereby increasing the rate of oocyte fertilization of the goat nuts.