Abstract
The scaling of geothermal wells arising from formation of calcium carbonate is one of the major problems associated with the utilization of geothermal energy. A novel eco-friendly biological-based approach for geothermal well descaling was proposed. Thermophilic bacterial strains were isolated from geothermal areas in Taiwan and were evaluated for their calcium adsorption efficiency under the extreme conditions. Among the eight strains isolated, Tepidimonas fonticaldi AT-A2 isolated from Antun Hot Spring, Hualien showed the highest calcium adsorption capacity. The calcium adsorbing activity of T. fonticaldi AT-A2 was mainly associated with the extracellular proteins and the maximum calcium adsorption capacity (1.94gCa/g protein) was obtained at pH 10, 150°C and 1atm pressure. This calcium adsorption efficiency is much higher than that of metallothioneins and other bacterial extracellular proteins. The excellent calcium adsorption efficiency of the AT-A2 proteins indicates the potential for their applications in biological geothermal well descaling.
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