Abstract

1. Substitution by different anions for chloride in physiological salt solutions leads to binding between Ca 2+ and the anion. Experiments designed to test effects of Cl − therefore often show mixed effects of changes in Cl − and Ca 2+ activity. 2. This mixed effect is demonstrated in neurons of the snail, Helix pomatia: current—voltage characteristics and membrane potential are described during reduction of extracellular Cl − using different anions as substitutes. Methylsulphate is concluded to be the best substitute in this preparation. 3. The association constants for the binding of Ca 2+ to a number of anions is given.

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