Abstract
This review updates the imaging of free cytosolic calcium with the chemiluminescent aequorins. Basic principles of chemiluminescence are discussed and the biochemistry of aequorins is briefly described. The review provides practical tips on handling and microinjecting aequorins and describes available ultra low light imaging systems. It is argued that aequorin-based calcium imaging is the method of choice for exploratory studies, since it is extremely sensitive, can detect a broad range of calcium concentrations, and allows for continuous recording during long periods of time. However, fluorescent methods are needed to attain high spatial resolution.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
