Abstract
The use of washed brushite, CaHPO 4, as a filter aid permitted the flow rates of calcium hydroxyapatite (HAP) columns to be made reliable and high. Regeneration of the column contents yielded pure, crystalline HAP, which could be mixed with fresh brushite and reused. Column fractionation over HAP-brushite with a phosphate gradient was used as a second ion exchange step in protein purification, the first step being fractionation over DEAE-cellulose. In two examples discussed, HAP-brushite fractionation in the presence of an effector molecule, a molecule for which the enzyme of interest had a specific affinity, gave marked improvement of resolution.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
