Abstract
Pectate lyase (PL) is the principal or sole enzyme responsible for maceration of plant tissue caused by most strains of soft-rotting pseudomonads. Production of PL in four out of 25 Pseudomonas fluorescens (or P. marginalis) strains examined was not induced by the enzyme substrate, polygalacturonate (PGA), but was induced by Ca 2+ . These four strains produced 10 times more PL in medium containing 1 mM CaCl, than in one containing no CaCl, supplement. Over 86% of total PL produced by these strains in CaCl 2 -supplemented medium was excreted into the culture fluid. Only a small portion (13%) of total PL produced by these strains in CaCl 2 -deficient medium was detected in the extracellular fraction [...]
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