Abstract
Human muscle cells obtained from biopsy specimens were grown in a primary culture system and electrophysiologically studied. Whole cell patch-clamp recordings revealed the presence of two types of calcium currents: (i) a low-threshold (−60 mV) one (I Ca,T) with fast activation and inactivation kinetics (time-to-peak: 39 ms at −30 mV); and (ii) a high-threshold (−10 mV) one (I Ca,L) with slower kinetics (time-to-peak: 550 ms at 20 mV). These two types of calcium currents could be also distinguished by their pharmacological characteristics since I Ca,L was sensitive to the antagonist and agonist dihydropyridine derivatives contrary to I Ca,T which was completely resistant to these compounds. These functional calcium channels existed both in normal and Duchenne dystrophic (DMD) human skeletal muscle cells in culture. We discuss a possible role of these two types of calcium channels in the myoplasmic calcium accumulation observed in the Duchenne muscular dystrophy.
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