Calcium currents in acutely isolated stellate and pyramidal neurons of rat entorhinal cortex

Abstract

Calcium currents were studied in morphologically identified pyramidal and stellate neurons acutely isolated from layer II/III of rat entorhinal cortex, using the whole-cell patch-clamp configuration. The peak amplitude of high-voltage activated current (HVA) measured at +10 mV was not different in both neuron populations with 0.94±0.08 nA for pyramidal and 1.03±0.08 nA for stellate cells. Stellate neurons had a larger capacitance (14.4±1.1 pF) than pyramidal neurons (9.6±0.8 pF), indicating a 50% larger cell surface. Most striking was the difference between the current density in stellate (79±8 pA/pF) versus pyramidal neurons (113±13 pA/pF). The potential of half maximal inactivation was not different: −37±2 mV (pyramidals) and −37±3 mV (stellates). Half of the cells contained a low-voltage activated calcium current (LVA) with a peak amplitude that was twice as large in stellate as in pyramidal neurons (0.21±0.04 nA resp. 0.11±0.03 nA; at −50 mV). In contrast to the HVA component, the current density of the LVA component was not different between cell types (13±3 pA/pF vs. 13±2 pA/pF). This implies that the relative abundance of LVA and HVA currents in stellate and pyramidal neurons is different which could result in different firing characteristics. The potential of half maximal LVA inactivation was −88±4 mV (pyramidals) and −85±3 mV (stellates). The slope of the voltage dependent steady state inactivation was steeper in stellate (7±1 mV) than in pyramidal cells (10±2 mV).