Calcium/calmodulin-dependent nitric oxide synthase activity in the CNS of Aplysia californica: Biochemical characterization and link to cGMP pathways

Abstract

We characterized enzymatic activity of nitric oxide synthase (NOS) in the central nervous system of Aplysia californica, a popular experimental model in cellular and system neuroscience, and provided biochemical evidence for NO-cGMP signaling in molluscs. Aplysia NOS (ApNOS) activity, determined as citrulline formation, revealed its calcium-/calmodulin-(Ca/CaM) and NADPH dependence and it was inhibited by 50% with 5 mM of W7 hydrochloride (a potent Ca/CaM-dependent phosphodiesterase inhibitor). A representative set of inhibitors for mammalian NOS isoforms also suppressed NOS activity in Aplysia. Specifically, the ApNOS was inhibited by 65–92% with 500 μM of l-NAME (a competitive NOS inhibitor) whereas d-NAME at the same concentration had no effect. S-Ethylisothiourea hydrobromide (5 mM), a selective inhibitor of all NOS isoforms, suppressed ApNOS by 85%, l- N6-(1-iminoethyl)lysine dihydrochloride ( l-NIL, 5 mM), an iNOS inhibitor, by 78% and l-thiocitrulline (5 mM) (an inhibitor of nNOS and iNOS) by greater than 95%. Polyclonal antibodies raised against rat nNOS hybridized with a putative purified ApNOS (160 kDa protein) from partially purified central nervous system homogenates in Western blot studies. Consistent with other studies, the activity of soluble guanylyl cyclase was stimulated as a result of NO interaction with its heme prosthetic group. The basal levels of cGMP were estimated by radioimmunoassay to be 44.47 fmol/μg of protein. Incubation of Aplysia CNS with the NO donors DEA/NONOate (diethylammonium (Z)-1-( N, N-diethylamino) diazen-1-ium-1,2-diolate – 1 mM) or S-nitroso- N-acetylpenicillamine (1 mM) and simultaneous phosphodiesterase inhibition with 3-isobutyl-1-methylxanthine (1 mM) prior to the assay showed a 26–80 fold increase in basal cGMP levels. Addition of ODQ (1H-[1,2,4]oxadiazolo[4,3-a] quinoxaline-1-one – 1 mM), a selective inhibitor of soluble guanylyl cyclase, completely abolished this effect. This confirms that NO may indeed function as a messenger in the molluscan CNS, and that cGMP acts as one of its effectors.