Abstract
Ca 2+ binding to two kinds of brain membranes is studied. The samples include neuronal-enriched plasma membranes from newborn rat brain and myelin from adult rat brain. Two or more apparent Ca 2+ binding sites are detected in the two membranes. At 5 · 10 −2 M Ca 2+ myelin binds 830 and plasma membranes 520 nmoles of Ca 2+ per mg protein. The Ca 2+-binding component is examined using enzymes and extraction but the treatment reduces binding by only 50%, which is attributable to phospholipids and neuraminic acid. Incubation with pronase appears to increase Ca 2+ binding suggesting unmasking of Ca 2+-binding sites. Monovalent cations (Na +, K +) decrease Ca 2+ binding non-competitively while Mg 2+ shows competitive inhibition. 5-Hydroxytryptamine and ATP significantly decrease Ca 2+ binding to plasma membranes but not to myelin.
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