Calcium Binding Protein Immunoreactivity in Nucleus Rotundus in a Reptile, Caiman crocodilus

Abstract

Nucleus rotundus is a prominent nucleus in the dorsal thalamus of nonmammalian amniotes. In one group of reptiles, Caiman crocodilus, previous studies have identified three parts of this neuronal aggregate. The central portion, the rotundal core, which receives visual input from the midbrain and projects to a restricted portion of the telencephalon, contains relay cells only. Previous examinations using Nissl morphology indicated that neurons of the rotundal core were not a homogeneous population of cells. The present investigation utilized another methodology to examine cell populations within the rotundal core, immunoreactivity to the calcium binding proteins, calbindin/calretinin and parvalbumin. Light microscopic observations revealed the following features. First, calbindin/calretinin immunoreactive neurons and parvalbumin immunoreactive neurons were present in the rotundal core. Of these two antibodies, immunoreactivity to calbindin/calretinin was much more robust and calbindin/calretinin immunoreactive neurons were more numerous than parvalbumin cells. Second, neurons immunoreactive to either calbindin/calretinin or parvalbumin were not homogeneous but comprised several populations based on perikaryal shape and size and neuronal process morphology. These results are compared with similar data in other amniotes.