CALCIUM AND PHOSPHORUS CONTENT OF THE COMPACT BONE IN RATS WITH ALLOXAN DIABETES

Abstract

Introduction: Most of the socio-economic importance of diabetes is an early disability of patients suffering from it. Among the chronic complications of diabetes, there are also abnormal changes in bone tissue. Aim: The aim of our work was to study the changes in the amount of calcium and phosphorus (the main inorganic constituents of bone) in a compact bone tissue in alloxan-induced diabetes in rats at different stages of the disease. Materials and Methods: The experiment was conducted on 70 white mongrel male rats weighing 180 to 220 g. Using the method of atomic emission spectroscopy with inductively coupled plasma we determined the content of calcium and phosphorus in the samples - femoral shafts weighting 5 mg. In homogenates of samples, we determined the activity of alkaline phosphatase. The entire list of measurements was repeated at 7, 14, 21 and 28 days of experiment. Results: The work revealed the decreases in the content of calcium at 7, 14 and 28 days of observation and phosphorus throughout the experiment with maximum changes on the 7th day of the experiment: 25% (p = 0.00002) and 15% (p = 0.002), respectively, compared with control values (median: 1.9 mg -calcium, 0.73 mg - phosphorus). There was a strong direct correlation between calcium and phosphorus (r = 0.086; p <0.01) and an inhibition of activity of alkaline phosphatase on day 28 of the experiment. Conclusions: For the first time, we show that alloxan diabetes in rats is accompanied by a decrease in phosphorus and calcium content of the compact bone with a maximum change at day 7 of the experiment. There are phase changes in the activity of alkaline phosphatase characterized by an increase at day 7 and inhibition by day 28 of the experiment.