Calcium alleviation of hydrogen and aluminum inhibition of soybean root extension from limed soil into acid subsurface solutions

Abstract

Alleviation by calcium (Ca) of inhibition of soybean [Glycine max (L.) Merr. cv. ‘Ransom'] root elongation by hydrogen (H) and aluminum (Al) was evaluated in a vertical split‐root system. Roots extending from a limed and fertilized soil compartment grew for 12 days into a subsurface compartment containing nutrient solution with treatments consisting of factorial combinations of either pH (4.0, 4.6, and 5.5) and Ca (0.2, 2.0, 10, and 20 mM), Al (7.5, 15, and 30 μM) and Ca (2.0,10, and 20 mM) at pH 4.6, or Ca (2, 7, and 12 mM) levels and counter ions (SO4 and Cl) at pH 4.6 and 15 μM Al. Length of tap roots and their laterals increased with solution Ca concentration and pH value, but decreased with increasing Al level. Length of both tap and lateral roots were greater when Ca was supplied as CaSO4 than as CaCl2, but increasing Ca concentration from 2 to 12 mM had a greater effect on alleviating Al toxicity than Ca source. In the absence of Al, relative root length (RRL) of tap and lateral roots among pH and Ca treatments was related to the Ca:H molar activity ratio of solutions (R2≥0.82). Tap and lateral RRL among solutions with variable concentrations of Al and Ca at pH 4.6 were related to both the sum of the predicted activities of monomeric Al (R2≥0.92) and a log‐transformed and valence‐weighted balance between activities of Ca and selected monomeric Al species (R2≥0.95). In solutions with 15 μM Al at pH 4.6, response of tap and lateral RRL to variable concentrations of CaSO4 and CaCl2 were related to predicted molar activity ratios of both Ca:Al3+ (R2≥0.89) and Ca:3 monomeric Al (R2≥0.90), provided that AISO4 and AI(SO4)2 species were excluded from the latter index. In all experiments H and Al inhibited length of lateral roots more than tap roots, and a greater Ca:H or Ca:Al concentration ratio was required in solutions to achieve similar RRL values as tap roots.