Calcitonin Gene-Related Peptide Immunoreactive Cells and Fibers in Forebrain Vocal and Auditory Nuclei of the Budgerigar (Melopsittacus undulatus)

Abstract

The distributions of calcitonin gene-related peptide (CGRP) immunoreactive neurons and fibers were mapped within forebrain vocal control and auditory nuclei of a vocal learning psittacine species, the budgerigar (Melopsittacus undulatus). Immunoreactivity was exhibited by telencephalic nuclei previously associated with vocal control pathways on the basis of both tract tracing studies and gene mapping: the central nucleus of the anterior archistriatum (AAc), central nucleus of the lateral neostriatum (NLc), magnocellular nucleus the lobus parolfactorius (LPOm), the oval nucleus of the ventral hyperstiratum (HVo) and the medial division of the oval nucleus of the anterior neostriatum (NAom). The main body of NAo also contained an exceptionally high density of immunoreactive fibers. In contrast to the condition in oscine songbirds, CGRP-positive neuronal somata were not present in any telencephalic vocal control nucleus. CGRP-positive somata were present, however, in diencephalic cell groups that included the shell region of the nucleus ovoidalis (Ov), the nucleus dorsolateralis posterior (DLP) and a region of the ventral thalamus that was retrogradely labeled by tracer deposits into HVo and AAc. CGRP immunoreactive fibers were observed within auditory areas of the telencephalon including Field L and the neostriatum intermedium pars dorsolateralis. The likely sources of these fibers are CGRP-positive neurons within the Ov shell and DLP.