Abstract
Exposure of small slabs of presoftened tooth enamel to human bloods, synovial fluids, and salivary components caused a marked restoration of enamel hardness. The increase of hardness appeared to be related to the re-formation of hydroxyapatite. The procedure thus provides a method of measuring the calcification ability of a fluid. After implantation of the slabs subdermally in guinea pigs for 8 days, in vivo recalcification to the extent of 74–120 Knoop hardness units (KHN) occurred. Parotid, submaxillary and whole saliva had similar and strong calcification properties, indicated by rehardening to the extent of 100 KHN in 4–6 hr. Human serums and synovial fluids had modest but definite rehardening characteristics of the order of 30 KHN at 6 hr. The method appears to offer promise as an aid in the study of abnormal calcification and decalcification processes.
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