Abstract
Cardiovascular disease is the leading cause of death in diabetes mellitus patients. Medial calcification in diabetic patients is a strong predictor of cardiovascular mortality independent of other factors. The role of glucose metabolism in vascular smooth muscle (VSMC) osteochondrogenic differentiation has recently been highlighted and is an area of growing interest. Calcifying VSMC have increased glucose uptake and glycolysis. To further investigate the role of metabolism in vascular calcification we cultured primary mouse aortic smooth muscle cells (MASMC) in high phosphate (3 mM) medium to induce calcification, calcium content was determined by a modified O-Cresolphtalein method and normalised to total protein. Inhibiting glycolysis using 2-deoxyglucose (10 µM) decreased high phosphate induced calcification by ~30% (p
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