Abstract
The paper deals with a study of the deposition of calcium phosphate in the form of apatite crystallites inside collagen fibres. By treating collagen fibres with a suspension of precipitated calcium phosphate, an excellent precipitation of hydroxyapat-ite inside the collagen was obtained. The X-ray diffraction pattern of the treated specimen showed a striking similarity to that of a section of normal bone. The lines were sharper in the pattern of the treated specimen indicating a large particle size in the precipitate. Addition of citric acid in small amounts was found to affect the particle size and the X-ray pattern was found to correspond more closely to the bone pattern. Increased addition of citric acid inhibited the deposition of apatite while a still larger concentration of citric acid resulted in the crystallization of calcium citrate itself. The presence of other salts like calcium carbonate and calcium chloride suppressed the deposition of apatite. In the case of calcium fluoride a mixed crystallization of fluoride and apatite took place if the calcium fluoride was present in equal concentration, while the presence of excess of calcium fluoride was found to completely suppress the deposition of both apatite and fluoride.
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