Calcification and decalcification rates in vitro. A new technique.

Abstract

SummaryA sedimentation column apparatus was developed in which the rates of calcification and decalcification of bone and dentin could be followed readily and quantitatively. The method utilizes a long column with guiding channel and can be rotated inside an air thermostat. The rates of calcification and decalcification are measured by following the sedimentation velocities of the specimen in the column of calcifying or decalcifying solutions with time. The bone specimens used are very small. Typical results obtained in decalcification with EDTA and lactic acid, and of calcification rates of rachitic cartilage are presented and discussed.