Abstract
Objectives To investigate the relationship between polymorphisms of calcitonin-related peptide gene II (beta-calcitonin gene-related peptide (βCGRP), CALCB) and serum CGRP levels in salivary adenoid cystic carcinoma. Materials and Methods Using the polymerase chain reaction (PCR) technique, the full-length amplification and genotype analysis of CALCB genes were performed in 39 patients with adenoid cystic carcinoma of salivary gland and 158 normal controls. The gene frequencies of major genotype of CALCB in adenoid cystic carcinoma of salivary gland and normal control group were analyzed. Enzyme-linked immunosorbent assay (ELISA) was used to evaluate serum calcitonin gene-related peptide (CGRP) and its concentration of alpha and beta subtypes. Results Univariate logistic regression analysis showed that the CALCB rs2839222 T/T genotype was closely related to the occurrence of salivary adenoid cystic carcinoma, with a correlation coefficient of 3.89. Conclusions The serum CGRP concentration in the salivary adenoid cystic carcinoma group was 1.56 times that of the normal control group. The αCGRP subtype was significant, which was 3.02 times that of the normal control. The polymorphism of βCGRP gene is associated with genetic susceptibility to salivary adenoid cystic carcinoma, and serum CGRP and βCGRP can be used as novel markers of salivary adenoid cystic carcinoma.
Highlights
Salivary adenoid cystic carcinoma (SACC) is a common malignant tumor of the frontal face of the oral cavity, the pathogenesis of which is still unclear, and the biological markers closely related to the occurrence and development of SACC are currently the hot spot of clinical research
Disease Markers validated in studies on the prognostic relevance of periodontitis and coronary heart disease [1], and some studies have found an association between serum markers and the pathogenesis of certain diseases [2, 3]
We hypothesized that calcitonin gene-related peptide (CGRP) gene and homologous gene polymorphisms and serum levels are associated with salivary adenoid cystic carcinoma
Summary
Salivary adenoid cystic carcinoma (SACC) is a common malignant tumor of the frontal face of the oral cavity, the pathogenesis of which is still unclear, and the biological markers closely related to the occurrence and development of SACC are currently the hot spot of clinical research. The main physiological functions of CALCB are related to the nervous system, immune system, skin system, and cardiovascular systems and are involved in a variety of physiological processes such as neurotrophy, skin immunomodulation, vasodilation, and antigen presentation. It can be used as an indicator of disease severity and therapeutic efficacy and can determine the prognosis of the disease, which has potential clinical application in treatment [4]. We hypothesized that CGRP gene and homologous gene polymorphisms and serum levels are associated with salivary adenoid cystic carcinoma. It is expected that the relationship between CGRP gene polymorphism and its expression and SACC can be explored to provide reference significance for early clinical diagnosis
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