Abstract
BackgroundThe spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. In this study, the new epithelial ovarian cancer cell line CAISMOV24 was described and compared with its original low-grade serous ovarian carcinoma.MethodsThe in vitro culture was established with cells isolated from ascites of a 60-year-old female patient with recurrent ovarian cancer. The CAISMOV24 line was assessed for cell growth, production of soluble biomarkers, expression of surface molecules and screened for typical mutations found in serous ovarian carcinoma. Additionally, comparative genomic hybridization was employed to compare genomic alterations between the CAISMOV24 cell line and its primary malignant cells.ResultsCAISMOV24 has been in continuous culture for more than 30 months and more than 100 in vitro passages. The cell surface molecules EpCAM, PVR and CD73 are overexpressed on CAISMOV24 cells compared to the primary malignant cells. CAISMOV24 continues to produce CA125 and HE4 in vitro. Although the cell line had developed alongside the accumulation of genomic alterations (28 CNV in primary cells and 37 CNV in CAISMOV24), most of them were related to CNVs already present in primary malignant cells. CAISMOV24 cell line harbored KRAS mutation with wild type TP53, therefore it is characterized as low-grade serous carcinoma.ConclusionOur results corroborate with the idea that genomic alterations, depicted by CNVs, can be used for subtyping epithelial ovarian carcinomas. Additionally, CAISMOV24 cell line was characterized as a low-grade serous ovarian carcinoma, which still resembles its primary malignant cells.
Highlights
The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing
Our results showed that CAISMOV24 cell line had arisen alongside the accumulation of genomic alterations, most of them were related to copy number variation (CNV) already present in the primary malignant cells
In this study, the CAISMOV24 cell line was compared to its primary malignant cells
Summary
The spontaneous immortalization of primary malignant cells is frequently assigned to their genetic instability during in vitro culturing. Cancer antigen 125 (CA125) and human epididymis protein 4 (HE4) have been detected in the serum of ovarian cancer patients Together, they enhance the sensitivity and specificity for the diagnosis of the disease [7, 8]. Molecular characteristics of tumors have become the new standard classification for clinical pathology In this concern, methods such as comparative genomic hybridization allow the detection of deletions and duplications of genomic segments, known as copy number variation (CNV). Methods such as comparative genomic hybridization allow the detection of deletions and duplications of genomic segments, known as copy number variation (CNV) This method has been used to evaluate differences between cancer histotypes and putative target driver genes in EOC [12, 13]
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