Abstract
Protoplast culture following polyethylene glycol-induced fusion resulted in the regeneration of somatic hybrid plants between Caipira sweet orange (Citrus sinensis L. Osbeck) and Rangpur lime (C. limonia L. Osbeck). The plants were confirmed as somatic hybrids by leaf morphology, chromosome number and RAPD profile. All regenerated plants were tetraploid (2n = 4x = 36), with intermediate leaf morphology and complementary RAPD banding profile of both parents. This combination may be useful as a rootstock for the citrus industry in Southeastern Brazil since this somatic hybrid could combine the drought tolerance and vigor of Rangpur lime with the blight tolerance of Caipira sweet orange.
Highlights
The State of São Paulo, in southeastern Brazil, accounts for one third of the world’s orange production (Neves and Boteon, 1998)
We report the production of somatic hybrids between Rangpur lime and Caipira sweet orange
The number of embryos induced on this medium is very low in spite of several reports showing that sucrose is the carbon source for citrus somatic embryogenesis (Kobayashi and Ohgawara, 1988; Grosser and Gmitter Jr., 1990; Ohgawara et al, 1994; Grosser et al, 1996; Mourão Filho et al, 1996)
Summary
The State of São Paulo, in southeastern Brazil, accounts for one third of the world’s orange production (Neves and Boteon, 1998). We report the production of somatic hybrids between Rangpur lime and Caipira sweet orange. An embryogenic cell suspension culture derived from Caipira sweet orange
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