Abstract
Fringed sagewort (Artemisia frigida Willd., Compositae family) is a well-known medicinal plant in Asian medical systems. Fifty-nine hydroxycinnamates and flavonoids have been found in A. frigida herbs of Siberian origin by high-performance liquid chromatography with diode array and electrospray triple quadrupole mass detection (HPLC-DAD-ESI-QQQ-MS). Their structures were determined after mass fragmentation analysis as caffeoylquinic acids, flavone O-/C-glycosides, flavones, and flavonol aglycones. Most of the discovered components were described in A. frigida for the first time. It was shown that flavonoids with different types of substitution have chemotaxonomic significance for species of Artemisia subsection Frigidae (section Absinthium). After HPLC-DAD quantification of 16 major phenolics in 21 Siberian populations of A. frigida and subsequent principal component analysis, we found substantial variation in the selected compounds, suggesting the existence of two geographical groups of A. frigida. The antioxidant activity of A. frigida herbal tea was determined using 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH•) and hydrophilic/lipophilic oxygen radical absorbance capacity (ORAC) assays and DPPH•-HPLC profiling, revealing it to be high. The effect of digestive media on the phenolic profile and antioxidant capacity of A. frigida herbal tea was assessed under simulated gastrointestinal digestion. We found a minor reduction in caffeoylquinic acid content and ORAC values, but remaining levels were satisfactory for antioxidant protection. These results suggest that A. frigida and its food derivate herbal tea could be recommended as new plant antioxidants rich in phenolics.
Highlights
Traditional healing treatments refer to the collective knowledge, skills and practices that are based on the theories, values and personal experiences developed and used by indigenous people of different cultures to improve health, avoid and reduce diseases and their spread, or as complete curesAntioxidants 2019, 8, 307; doi:10.3390/antiox8080307 www.mdpi.com/journal/antioxidantsTraditional healing treatments refer to the collective knowledge, skills and practices that are values and personal experiences developed and used by indigenous people2 of of different cultures to improve health, avoid and reduce diseases and their spread, or as complete cures of both physical and mental health conditions [1,2]
Detected compounds were identified by their retention times and ultraviolet spectral (UV) and mass spectrometric data (Figure S1) by comparison with reference standards (Figure S2) and literature data
Caffeoylquinic acids are widely distributed in species of the genus Artemisia [21], but their presence in A. frigida has been demonstrated for the first time here
Summary
Traditional healing treatments refer to the collective knowledge, skills and practices that are based on the theories, values and personal experiences developed and used by indigenous people of different cultures to improve health, avoid and reduce diseases and their spread, or as complete cures. North American samples of A. frigida were characterized by the ability to accumulate derivatives of luteolin and heptahydroxyflavone, whereas the glycosides of tricetin were found in specimens from China and Mongolia. We decided to characterize caffeoylquinic acids and flavonoids of A. frigida using high-performance liquid chromatography (HPLC) with both diode array detection (DAD) and electrospray ionization triple quadrupole mass spectrometry (ESI-QQQ-MS) to compare the cumulative content of flavonoids and caffeoylquinic acids in the herb of the Siberian populations of fringed sagewort, according to habitat, by HPLC-DAD quantification. Because antioxidant properties of A. frigida herbal tea may be an important means of protecting the gastrointestinal tract itself, the total antioxidant capacity of non-treated herbal tea and herbal tea after gastric and intestinal phases from sagaan aya tea was assessed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
