Abstract
Background and aim of study: Cardioplegic arrest remains the method of choice for myocardial protection in cardiac surgery. Caffeic acid phenethyl ester (CAPE) prevents lipid peroxidation induced by ischemia-reperfusion injury and has a potent antioxidant property. We investigated the advantages of CAPE supplemented cardioplegic solution (St. Thomas’ Hospital cardioplegic solution No.: 2) on the antioxidant defense system of myocardium against ischemia-reperfusion injury. Material and methods: Isolated rat hearts were mounted on a nonrecirculating type of Langendorff apparatus. The hearts were arrested for 60 min with cardioplegic solution given at 20-min intervals and then reperfused for 15 min. The hearts were divided into three groups. Cold saline (0.9%, 4 °C) in group 1, St. Thomas’ Hospital solution in group 2 and CAPE added St. Thomas’ Hospital solution in group 3 were used as the cardioplegic solution. Krebs–Henseleit buffer solution was used for reperfusion. The tissues were examined biochemically for oxidative stress. Results: Significant differences among the three groups existed in tissue myeloperoxidase (MPO), catalase (CAT), Na +–K + ATPase activity and in the concentrations of malonydealdehyde (MDA) and 3-nitrotyrosine (3-NT). Group 2 showed significant changes in MPO ( P = 0.04), Na +–K + ATPase enzyme activity ( P = 0.02) and the levels of MDA ( P = 0.004) and 3-NT ( P = 0.01) in comparison with group 1. Group 3 efficiently reduced MDA levels ( P = 0.004) and also led to significant decrease in levels of MPO ( P = 0.006), 3-NT ( P = 0.01) and Na +–K + ATPase activity ( P = 0.01) and increase in the level of CAT ( P = 0.004) in comparison with group 1. Significant changes were also found in the levels of MDA ( P = 0.03), MPO ( P = 0.04) and CAT ( P = 0.009) in comparison between groups 2 and 3. Conclusions: We demonstrated that the administration of CAPE into cardioplegic solutions improves the antioxidant defense system of rat heart during the ischemia-reperfusion injury.
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