Abstract
Abstract—Haloarchaeal strains from solar salterns of Ribandar and Siridao, Goa, India were tested for tolerance to cadmium. The four strains selected named as BBK2, BK6, BS2 and BS17 belonged to different genera of halophilic archaea. An initial screening for various heavy metals [Zn, Cu, Ag, Co and Cd] was performed. Cadmium (Cd) concentrations of 0.5, 1, 2 and 4 mM Cd in complex and glucose synthetic media containing high sodium chloride concentrations were used. Growth was monitored by monitoring optical density (O.D.) values for each of the sample cultures in 24 hr intervals for an incubation period of 5-7 days. Pigmentation and growth was observed in test cultures in complex nutrient media containing cadmium. Interesting results for pigmentation scans of the tested strains having varying peak intensities and exhibiting spectrophotometric profiles associated with typical haloarchaeal pigments were obtained. Correlation between peak intensities of the tested strains and the amount of cadmium in test media was also observed. This investigation shows cadmium tolerance in haloarcheal strains.
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