Abstract
The effect of Cd2+ (4ppm) and a combination of Cd2+ with the non-inhibitory levels of metals like Ca2+ (150ppm), Zn2+ (2ppm), and Hg2+ (0.04ppm) on the uptake of nitrate by Anacystis nidulans IU 625 (ATCC 27144) was investigated. The NO3- uptake kinetics was studied by monitoring short-term uptake rates at different NO3- levels (1-20mM). The values for Vmax (0.8μmol/μg protein/hr) and half saturation constant for uptake (Km 3.03mM) were obtained employing the double reciprocal plot. A 4ppm level of Cd2+ inhibited 50% of the NO3- uptake rate (at saturating NO3- level) compared to Cd2+-less control. Cd2+ inhibition of NO3- uptake (Km 5.88mM) was alleviated as a result of competitive interaction by either Ca2+ (Km 4.34mM) or Zn2+ (Km 4.08mM). Hg2+ ions, however, interacted synergistically with Cd2+ (Km 12.5mM). There was no change in the uptake capacity (Vmax 0.8μmol/μg protein/hr) for NO3- under various treatments.
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