Abstract
The presence of glucose resulted in a two- to three-fold increase in levels of Cd2+accumulated by Saccharomyces cerevisiae after 5 h compared with those observed in the absence of glucose. However, time-dependent Cd2+ uptake continued in the absence of glucose over 5 h, resulting in an appreciable increase in cellular Cd2+levels. Substantial K+ efflux but little Mg2+ and negligible Ca2+release was observed. Cell fractionation revealed that the bulk of intracellular Cd2+ was located in the vacuolar (25%) and bound (60%) fractions. Accumulation of Cd2+ ions impacted most noticeably on K+ rather than Mg2+ levels in intracellular compartments. Cytoplasmic and particularly vacuolar K+ levels decreased as Cd2+ sequestration continued resulting in increased extracellular levels. In contrast, corresponding intracellular Mg2+ pools were only modestly affected with a slight increase and decrease observed in the cytoplasmic and vacuolar fractions respectively. However, levels of bound Mg2+ decreased in response to continued Cd2+ accumulation.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Industrial Microbiology and Biotechnology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
