Abstract
Temporal increases in intracellular [Ca2+] are now recognized to be key triggers for a wide range of important physiological events in eukaryotic cells. In mammalian cells, signal-induced Ca2+-elevations have been found to be of a pulsatile nature and Ca2+spikes display a high degree of spatiotemporal complexity. In plant cells a similar picture is beginning to emerge. To investigate the occurrence of pulsatile Ca2+signals in plant cells we studied alterations of [Ca2+] in the tip region of pollen tubes from poppy (Papaver rhoeas). Time-Resolved Laser Scanning Confocal Microscopy of pollen tubes microinjected with the Dextran-linked Ca2+-indicator dyes Calcium Green or Indo-1 revealed that highly regular Ca2+oscillations occur in these cells. We further demonstrate that artificial elevation of cytosolic Ca2+by photolysis of caged-Ca2+(Nitr-5) can trigger the onset of oscillations.
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