Abstract
Addition of micromolar Ca2+ to electropermeabilized human platelets which had been pre-labelled with [3H]arachidonate causes release of 3H only when millimolar concentrations of a nucleoside triphosphate, e.g. ATP, are present in the incubation medium. Addition of millimolar Ca2+ in the absence of ATP, or preincubation with ATP before addition of micromolar Ca2+, fails to cause a significant increase in 3H release. Purine nucleotides are more effective than pyrimidine nucleotides in activating Ca2(+)-driven 3H release. This activation does not appear to involve phosphate transfer, since metabolically stable analogues of ATP, e.g. the beta gamma-imido analogue, are effective in promoting 3H release.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
