Abstract
Three methods of Ca2+ administration were used to influence the location of the grey crescent and the dorsal lip of blastopore in R temporaria eggs, ie a Ca2+ microinjection into the subcortical cytoplasm, egg pricking in high Ca2+ solutions and Ca2+ ionophore A23187 microinjection and application. The treatments all induced grey crescent and dorsal lip of blastopore formation near the Ca2+ administration site. Inositol trisphosphate injections gave similar results. Colchicine injections into the eggs inhibited the appearance of both natural and Ca(2+)-induced grey crescents.
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