Ca v1.2 calcium channels modulate the spiking pattern of hippocampal pyramidal cells

Abstract

Ca v1.2 L-type calcium channels support hippocampal synaptic plasticity, likely by facilitating dendritic Ca 2+ influx evoked by action potentials (AP) back-propagated from the soma. Ca 2+ influx into hippocampal neurons during somatic APs is sufficient to activate signalling pathways associated with late phase LTP. Thus, mechanisms controlling AP firing of hippocampal neurons are of major functional relevance. We examined the excitability of CA1 pyramidal cells using somatic current-clamp recordings in brain slices from control type mice and mice with the Ca v1.2 gene inactivated in principal hippocampal neurons. Lack of the Ca v1.2 protein did not affect either affect basic characteristics, such as resting membrane potential and input resistance, or parameters of single action potentials (AP) induced by 5 ms depolarising current pulses. However, CA1 hippocampal neurons from control and mutant mice differed in their patterns of AP firing during 500 ms depolarising current pulses: threshold voltage for repetitive firing was shifted significantly by about 5 mV to more depolarised potentials in the mutant mice ( p < 0.01), and the latency until firing of the first AP was prolonged (73.2 ± 6.6 ms versus 48.1 ± 7.8 ms in control; p < 0.05). CA1 pyramidal cells from the mutant mice also showed a lowered initial spiking frequency within an AP train. In control cells, isradipine had matching effects, while BayK 8644 facilitated spiking. Our data demonstrate that Ca v1.2 channels are involved in regulating the intrinsic excitability of CA1 pyramidal neurons. This cellular mechanism may contribute to the known function of Ca v1.2 channels in supporting synaptic plasticity and memory.